Location: Tropical Crops and Germplasm Research
Title: Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species Authors
|Witherup, Colby -|
|Ragone, Diane -|
|Wiesner-Hanks, Tyr -|
|Zuberi, M. Iqbal -|
|Zerega, Nyree J.C. -|
Submitted to: Applications in Plant Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 16, 2012
Publication Date: July 21, 2013
Citation: Witherup, C., Ragone, D., Wiesner-Hanks, T., Irish, B.M., Scheffler, B.E., Simpson, S.A., Zee, F.T., Zuberi, M., Zerega, N. 2013. Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species. Applications in Plant Sciences. DOI http://dx.doi.org/10.3732/apps.1200423. Interpretive Summary: Breadfruit, jackfruit and related species (Artocarpus spp., Moraceae) are important food sources and key species in tropical regions of the world. In order to effectively characterize, conserve and utilize these important plant genetic resources, new molecular markers have been developed. These markers are highly transferable between several related species and can be used to study evolutionary and domestication history as well as in estimating genetic diversity and gaps in existing Artocarpus spp. collections. The markers also have been shown to distinguish some breadfruit cultivars, which traditionally have been difficult to discern based on appearance. Markers developed are being used to characterize large international collections of Artocarpus spp. in Hawaii, Florida and Puerto Rico.
Technical Abstract: Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in three other Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. Nineteen microsatellite primers were tested on A. altilis, A. camansi, A. mariannensis, and A. altilis x mariannensis samples. Seven of those primers and three additional primers were tested on A. heterophyllus (jackfruit) samples. Three primer pairs showed amplification and polymorphism in two unlinked regions, yielding a total of 25 microsatellite loci. All loci are polymorphic for at least one species. The average number of alleles ranges from 2 – 9 within a species. These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and in establishing germplasm conservation strategies for breadfruit and jackfruit.