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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR APPROACHES FOR THE CHARACTERIZATION OF FOODBORNE PATHOGENS IN POULTRY

Location: Poultry Microbiological Safety Research

Title: Polymerase chain reaction detection of naturally occurring Campylobacter in commercial broiler chicken embryos

Authors
item Hiett, Kelli
item Cox, Nelson
item Rothrock, Michael

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 2, 2012
Publication Date: April 1, 2013
Citation: Hiett, K.L., Cox Jr, N.A., Rothrock Jr, M.J. 2013. Polymerase chain reaction detection of naturally occurring Campylobacter in commercial broiler chicken embryos. Poultry Science. 92(4):1134-1137.

Technical Abstract: Campylobacter, a foodborne pathogen closely associated with poultry, is recognized as a leading bacterial etiologic agent of human gastroenteritis in the United States. In this investigation, 2 trials were performed where tissues from seven, fourteen/fifteen, and nineteen day-old commercial broiler chicken embryos were tested for the presence of Campylobacter using both cultural methodology and polymerase chain reaction (PCR). Conventional cultural methods failed to detect Campylobacter from any samples tested during this investigation. Using a set of primers specific for the Campylobacter flagellin A short variable region (flaA SVR), Campylobacter DNA was amplified in 100%, 80%, and 100% of gastrointestinal tracts from seven, fifteen, and nineteen day-old embryos respectively in the first trial. Similarly, Campylobacter DNA was detected in 100%, 70%, and 60% of gastrointestinal tracts of seven, fourteen, and eighteen day-old embryos respectively in the second trial. In both trials, yolk sac, albumin, and liver/gall bladder samples from nineteen day-old embryos all failed to produce amplicons indicative of Campylobacter DNA. Subsequent DNA sequence analyses of the flaA SVR PCR products were consistent with the amplicon arising from Campylobacter. While a determination of whether the Campylobacter was living or dead within the embryos could not be made, these results demonstrate that Campylobacter-specific DNA is present within the gastrointestinal tract of broiler chicken embryos; however, the means by which it is present and the relative contribution to subsequent Campylobacter contamination of poultry flocks requires further investigation.

Last Modified: 8/29/2014
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