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United States Department of Agriculture

Agricultural Research Service

Research Project: Molecular Biology of Human Pathogens Associated with Food

Location: Produce Safety and Microbiology Research

Title: Top-down proteomic identification of bacterial protein biomarkers and toxins using MALDI-TOF-TOF-MS/MS and post-source decay

Author
item Fagerquist, Clifton

Submitted to: Reviews in Analytical Chemistry
Publication Type: Review Article
Publication Acceptance Date: February 28, 2013
Publication Date: April 2, 2013
Citation: Fagerquist, C.K. 2013. Top-down proteomic identification of bacterial protein biomarkers and toxins using MALDI-TOF-TOF-MS/MS and post-source decay. Reviews in Analytical Chemistry. 32(2):127-133. doi:10.1515/revac-2012-0035.

Technical Abstract: Matrix-assisted laser desorption/ionization time-of-flight-time-of-flight mass spectrometry(MALDI-TOF-TOF-MS)has provided new capabilities for the rapid identification of digested and non-digested proteins. The tandem (MS/MS) capability of TOF-TOF instruments allows precursor ion selection/isolation in the 1st TOF analyzer followed by post-source decay (PSD) or collision-induced dissociation (CID) followed by analysis of the sequence-specific fragment ions in the 2nd TOF analyzer. MS/MS allows specific fragment ions to attributed to the fragmentation of a specific precursor ion. A number of researchers have demonstrated top-down proteomic identification of non-digested proteins using MALDI-TOF-TOF-PSD as well as in-source decay (ISD). The current review discusses recent developments in the use of MALDI-TOF-TOF-MS/MS-PSD for top-down proteomic identification of protein biomarkers and toxins from un-fractionated bacterial cell lysates of foodborne pathogens. This approach provides great promise as a rapid method for identification and characterization of pathogenic microorganisms and their virulence factors.

Last Modified: 10/22/2014
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