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United States Department of Agriculture

Agricultural Research Service

Research Project: Intervention Strategies to Control and Prevent Disease Outbreaks Caused by Avian Influenza and Other Emerging Poultry Pathogens

Location: Exotic and Emerging Avian Viral Diseases Research Unit

Title: Avian Paramyxovirus serotypes circulating in wild bird populations of the Azov-Black Sea region of Ukraine in 2006-2011

Authors
item Muzyka, Denys -
item Pantin-Jackwood, Mary
item Stegniy, Borys -
item Afonso, Claudio

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 7, 2013
Publication Date: August 19, 2013
Citation: Muzyka, D., Pantin Jackwood, M.J., Stegniy, B., Afonso, C.L. 2013. Avian Paramyxovirus serotypes circulating in wild bird populations of the Azov-Black Sea region of Ukraine in 2006-2011 [abstract]. XVIII Congress of the World Veterinary Poultry Association, August 19-23, 2013, Nantes, France. p. 482.

Interpretive Summary: Over the past 40 years many different paramyxoviruses have been isolated from animals and birds. Paramyxovirus serotypes PMV-1, PMV-2, and PMV-3 are important because they can cause disease in poultry. The goal of our research was to study PMV-1 to PMV-9 paramyxovirus serotypes circulating in different types of wild birds in Ukraine, by virus sequencing and examination of some of their biological characteristics. During 2006-2011, virological investigations were conducted on 5091 specimens collected from wild birds belonging to 52 species (796 samples during the fall migration, 2769 during the winter, and 1526 during the spring migration, nesting and postnesting movements). Samples were collected in the Central and Eastern part of the Azov-Black Sea region of Ukraine. This region is a crossroad of transcontinental migration routes of wild birds from Siberia, Africa, Europe and Asia. Twenty different serotypes of paramyxoviruses (PMV-1, PMV-4, PMV-6 and PMV-7) were isolated from the samples. Infection prevalence of wild birds of different species was between 0,93-25,0%. PMV were mostly isolated from wild migratory waterfowl, except for PMV-4 which was isolated from starlings. The study of the biological properties of the PMV isolates showed different receptor specificity to erythrocytes of birds of 21 species and 4 species of mammals. Intracerebral pathogenicity index for all isolates was 0. Sequencing of the PMV-1 viruses determined that most belonged to class II of Newcastle disease virus, and only one to class I. Most class II viruses belonged to genotype I and II, and only one to genotype XIV. A XIV virus genotype isolated from a white-fronted goose in 2011 has the RRQKRF protein F cleavage site and is close to 2008 viruses that resulted in a number of disease outbreaks in West and Central Africa.

Technical Abstract: Over the past 40 years many different paramyxoviruses have been isolated from animals and birds. Paramyxovirus serotypes PMV-1, PMV-2, and PMV-3 are important because they can cause disease in poultry. The goal of our research was to study PMV-1 to PMV-9 paramyxovirus serotypes circulating in different types of wild birds in Ukraine, by virus sequencing and examination of some of their biological characteristics. During 2006-2011, virological investigations were conducted on 5091 specimens collected from wild birds belonging to 52 species (796 samples during the fall migration, 2769 during the winter, and 1526 during the spring migration, nesting and postnesting movements). Samples were collected in the Central and Eastern part of the Azov-Black Sea region of Ukraine. This region is a crossroad of transcontinental migration routes of wild birds from Siberia, Africa, Europe and Asia. Twenty different serotypes of paramyxoviruses (PMV-1, PMV-4, PMV-6 and PMV-7) were isolated from the samples. Infection prevalence of wild birds of different species was between 0,93-25,0%. PMV were mostly isolated from wild migratory waterfowl, except for PMV-4 which was isolated from starlings. The study of the biological properties of the PMV isolates showed different receptor specificity to erythrocytes of birds of 21 species and 4 species of mammals. Intracerebral pathogenicity index for all isolates was 0. Sequencing of the PMV-1 viruses determined that most belonged to class II of Newcastle disease virus, and only one to class I. Most class II viruses belonged to genotype I and II, and only one to genotype XIV. A XIV virus genotype isolated from a white-fronted goose in 2011 has the RRQKRF protein F cleavage site and is close to 2008 viruses that resulted in a number of disease outbreaks in West and Central Africa.

Last Modified: 10/25/2014
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