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United States Department of Agriculture

Agricultural Research Service

Research Project: INTERVENTION STRATEGIES TO CONTROL VIRAL DISEASES OF CATTLE Title: Circulating immune cell subpopulations in pestivirus persistently infected calves and non-infected calves varying in immune status.

Authors
item Falkenberg, Shollie
item Ridpath, Julia
item Bauermann, F -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 13, 2013
Publication Date: N/A

Technical Abstract: Circulating immune cell subpopulations in cattle representing varying stages of immune status categorized as; colostrum deprived (CD), receiving colostrum (COL), colostrum plus vaccination (VAC) and persistently infected with a pestivirus (PI) were compared. The PI calves were infected with a HoBi-like virus, a member of the Pestivirus genus similar to bovine viral diarrhea virus. All calves in the PI group tested positive for pestiviral protein using a commercial ELISA test, all other calves tested negative. Leukocyte (LEUK) and granulocyte populations were identified using flow cytometry based on forward and side scatter plots. Cell markers CD4, CD8, B cell, Gamma-delta (GDTCR) and CD14 were used to identify lymphocyte subpopulations. Differences (p<0.0001) were observed among the immune status groups in total LEUK, CD8, B cell, GDTCR and CD14 with the PI group being the lowest. The proportion of CD4 within total LEUK populations was significantly different (p<0.0001) and the PI group had a greater proportion of CD4 cells. These results suggest immune cell populations vary between immune status groups. The greatest differences were observed for the PI calves. These observations could provide insight into the association between immune cell populations and immune status.

Last Modified: 8/30/2014
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