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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETICS, POPULATION DYNAMICS, AND MYCOTOXIN PREVENTION IN PEANUT

Location: Peanut Research

Title: Clonality and sex impact aflatoxigenicity in Aspergillus populations

Authors
item Carbone, Ignazio -
item Horn, Bruce
item Olarte, Rodrigo -
item Moore, Geromy -
item Worthington, Carolyn -
item Monacell, James -
item Singhi, Rakhi -
item Stone, Eric -
item Hell, Kerstin -
item Chulze, Sofia -
item Barros, German -
item Wright, Graeme -
item Naik, Manjunath -

Submitted to: Fungal Genetics Conference/Asilomar
Publication Type: Abstract Only
Publication Acceptance Date: February 20, 2013
Publication Date: March 12, 2013
Citation: Carbone, I., Horn, B.W., Olarte, R.A., Moore, G.G., Worthington, C.J., Monacell, J.T., Singhi, R., Stone, E.A., Hell, K., Chulze, S.N., Barros, G., Wright, G., Naik, M.K. 2013. Clonality and sex impact aflatoxigenicity in Aspergillus populations. Fungal Genetics Conference. Pacific Grove, CA. March 12-17, 2013.

Interpretive Summary: none required.

Technical Abstract: Species in Aspergillus section Flavi commonly infect agricultural staples such as corn, peanuts, cottonseed, and tree nuts and produce an array of mycotoxins, the most potent of which are aflatoxins. Aspergillus flavus is the dominant aflatoxin-producing species in the majority of crops. Populations of aflatoxin-producing fungi may shift in response to: (1) clonal amplification that results from strong directional selection acting on a nontoxin- or toxin-producing trait; (2) disruptive selection that maintains a balance of extreme toxigenicities and diverse mycotoxin profiles; (3) sexual reproduction that results in continuous distributions of toxigenicity; or (4) female fertility/sterility that impacts the frequency of sexual reproduction. Population shifts that result in changes in ploidy or nuclear DNA composition (homokaryon versus heterokaryon) may have immediate effects on fitness and the rate of adaptation in subsequent fungal generations. We found that A. flavus populations with regular rounds of sexual reproduction maintain higher aflatoxin concentrations than predominantly clonal populations and that the frequency of mating-type genes is directly correlated with the magnitude of recombination in the aflatoxin gene cluster. Genetic exchange within the aflatoxin gene cluster occurs via crossing over between divergent lineages in populations and between closely related species. During adaptation, specific toxin genotypes may be favored and swept to fixation or be subjected to drift and frequency-dependent selection in nature. Results from mating experiments in the laboratory indicate that fertility differences among lineages may be driving genetic and functional diversity. Differences in fertility may be the result of female sterility, changes in heterokaryotic state, DNA methylation, or other epigenetic modifications. The extent to which these processes influence aflatoxigenesis is largely unknown, but is critical to understand for both fundamental and practical applications, such as biological control. Our work shows that a combination of population genetic processes, especially asexual/sexual reproduction and fertility differences coupled with ecological factors, may influence aflatoxigenicity in these agriculturally important fungi.

Last Modified: 10/21/2014
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