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ARS Home » Southeast Area » Florence, South Carolina » Coastal Plain Soil, Water and Plant Conservation Research » Research » Publications at this Location » Publication #293833

Title: Drought stress related gene expression patterns and sub-genome localization of five aquaporin genes in upland cotton (Gossypium hirsutum)

Author
item Bowman, Megan
item PARK, WONKEUN - Clemson University
item Bauer, Philip
item UDALL, JOSHUA - Brigham Young University
item PAGE, JUSTIN - Brigham Young University
item Scheffler, Brian
item Campbell, Benjamin - Todd

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2013
Publication Date: 6/30/2013
Citation: Bowman, M.J., Park, W., Bauer, P.J., Udall, J.A., Page, J.T., Scheffler, B.E., Campbell, B.T. 2013. Drought stress related gene expression patterns and sub-genome localization of five aquaporin genes in upland cotton (Gossypium hirsutum). In: Proceedings of the 2013 Joint Annual Meeting of the National Association of Plant Breeders and the Plant Breeding Coordinating Committee, Tampa, FL. June 2-5. 20.

Interpretive Summary:

Technical Abstract: As water resources become more and more limited, it is essential to understand how these changes in climate impact crop production. These environmental stresses are of particular concern for cotton, the world’s most important natural fiber and a significant crop economically for the Southeast United States. One approach to gain insight on the influence of water deficit on cotton is to study aquaporins, a large family of conserved proteins responsible for the regulation of cellular water uptake. Towards that end, using real time quantitative PCR (RT-qPCR), we have evaluated the level of transcript abundance of several genes from three sub families of aquaporins in the leaf and root tissues of tetraploid upland cotton cultivar Siokra-L23 during drought stress. Transcript amounts varied for each of the selected genes studied. Additionally, aquaporin sequence analysis from a wide range of diploid and tetraploid Gossypium germplasm and the use of a newly published PolyCat annotation pipeline have increased our knowledge of sub-genome localization and potential gene duplication of these important proteins in tetraploid cultivated upland cotton.