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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 5/18/2013 Publication Date: 5/23/2013 Citation: Pan, Y-B. 2013. Effectiveness of molecular techniques in identification of Erianthus hybrids [abstract]. International Workshop on Nobilisation of Erianthus, May 23-27, 2013, Fiji. Report available online: http://www.acp-srp.eu/sites/default/files/Rapport/cr%20fiji.pdf Interpretive Summary: Technical Abstract: It has been a challenge for sugarcane breeders to identify hybrid progeny from wild crosses involving wild relatives including Erianthus due to the tendency of sugarcane cultivars (Saccharum spp. hybrids) to self-pollinate during crossing. Selection of hybrid progeny on the basis of morphological traits alone is generally inefficient, but can be much more reliable using species- and genus-specific DNA markers. Two classes of PCR-based DNA markers, one targeting the 5S rDNA-intergenic transcribed spacer (ITS) region and the other targeting the microsatellite regions, are available. The 5S rDNA-ITS DNA markers include the degenerate primer pair PI/PII and Erianthus-specific primer pair Eri3/Eri4 that can effectively distinguish hybrids from either self- pollinated or outcrossed progeny of (sugarcane x Erianthus) crosses. The SSR DNA markers, once validated, are also useful tools in identifying (sugarcane x Erianthus) hybrid progeny using a capillary electrophoresis and fluorescence-labeling genotyping platform. The availability and detailed description of these two PCR-based molecular markers will help enhance the overall efficiency and productivity of Erianthus Noblization breeding process. |
