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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #294746
Title: Analysis of the aflatoxin AFB1 from corn by direct analysis in real time - mass spectrometry (DART-MS)

Author
item Busman, Mark
item LIU, JIHONG - Henan Agricultural University
item ZHONG, HONGJIAN - Henan Agricultural University
item Maragos, Chris

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/21/2013
Publication Date: 6/21/2013
Citation: Busman, M., Liu, J., Zhong, H., Maragos, C.M. 2013. Analysis of the aflatoxin AFB1 from corn by direct analysis in real time - mass spectrometry (DART-MS). Meeting Abstract.

Interpretive Summary:

Technical Abstract: Direct analysis in real time (DART) ionization coupled to a high resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of aflatoxins extracted from corn. Sample preparation procedure and instrument parameter settings were optimized to obtain sensitive and accurate determination of aflatoxins. 84:16 water acetonitrile extracts of corn were analyzed by DART-MS. The limits of detection and limit of quantitation (LOD and LOQ, respectively) for aflatoxin AFB1 were 4 and 10 ppb. Quantitative analysis was performed with the use of matrix-matched standards employing the 13C-labeled internal standard for AFB1. DART-MS of spiked corn extracts gave linear response of the range 4 ppb–1000 ppb. Good recoveries (94–110%) and repeatabilities (RSD 0.7–11.1%) were obtained at spiking levels of 20 and 100 µg/kg with use of an isotope dilution technique. Trueness of data obtained for AFB1 in maize by DART-MS was demonstrated by analysis of corn certified reference materials.