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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOP METHODS TO ASSESS AND IMPROVE POULTRY AND EGG QUALITY

Location: Quality and Safety Assessment Research Unit

Title: Surface plasmon resonance spectroscopy for analysis of influenza vaccines

Author
item Holser, Ronald

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 26, 2013
Publication Date: N/A

Technical Abstract: The hemagglutinin (HA) compounds are surface glycoproteins of a virus that can initiate an immune response from a host organism. Hemagglutinin and the related neuraminidase (NA) compounds are the basis for virus strain classification and have become part of the accepted HN taxonomy. These compounds may also be used to estimate the amount of viral material present in a vaccine. The ability to rapidly identify these components is important for quality control and testing of influenza vaccines. The current accepted test method, single radial immuno diffusion, relies on the immune response between HA and polyclonal antibodies. This test does not require sophisticated instrumentation but is criticized for variability [1]. An alternative test based on surface plasmon resonance (SPR) could provide more accurate results with minimal sample preparation and analysis time. Commercial SPR instruments use polarized light to excite a conductive surface and detect the resulting oscillations of free electrons. The presence of an adsorbed material produces a shift in the reflected signal and can be used to measure binding events such as antigen-antibody interactions [2]. Micro-arrays prepared with antibodies immobilized onto a conductive substrate would allow the detection and classification of the corresponding HN compounds. This technology was evaluated in silico to determine the limits of detection for this application with commercially available instrumentation.

Last Modified: 7/27/2014
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