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United States Department of Agriculture

Agricultural Research Service

Research Project: Methods for Rapid Identification and Functional Analysis of Fungi Causing Postharvest Decay of Pome Fruit

Location: Food Quality Laboratory

Title: The inhibitory effect of Bacillus megaterium on aflatoxin biosynthetic pathway gene expression in Aspergillus flavus

Authors
item Kong, Qing -
item Chi, Chen -
item YU, JIUJIANG

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2013
Publication Date: June 17, 2013
Citation: Kong, Q., Chi, C., Yu, J. 2013. The inhibitory effect of Bacillus megaterium on aflatoxin biosynthetic pathway gene expression in Aspergillus flavus. Meeting Abstract. p. 109.

Technical Abstract: Aspergillus flavus is one of the major fungal mold that colonize peanut in the field and during storage. The impacts to human and animal health and to economy in agriculture and commerce are significant since this mould produces the most potent natural toxins, aflatoxins, which are carcinogenic, mutagenic, immunosuppressive, and teratogenic. A strain of marine Bacillus megaterium isolated from the Yellow Sea of East China was evaluated for its inhibitory effect on aflatoxin formation through inhibiting aflatoxin pathway gene expression in A. flavus as demonstrated by genechip analysis. The results showed that aflatoxin accumulation in potato dextrose broth liquid medium and liquid minimal medium was almost totally (98%) inhibited by B. megaterium. The inhibitory efficiency on aflatoxin accumulation by B. megaterium, however, was shown slightly reduced (85%~87%) in two peanut varieties due to poor condition for optimum growth of B. megaterium on peanut plant compared with that in the liquid medium. The expression of most of the aflatoxin biosynthetic genes in the fungus was confirmed to be inhibited. Some of the gene targets (aflF, aflT, aflS, aflJ, aflX) for inhibition by B. megaterium were identified which can be used for controlling aflatoxin contamination in peanuts. The mechanism of inhibition is under investigation.

Last Modified: 9/29/2014
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