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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Analysis of Poultry-Associated Salmonella enterica to Identify and Characterize Properties and Markers Associated with Egg-Borne Transmission of Illness

Location: Egg Safety and Quality

Title: Serotype and Antimicrobial Resistance Patterns of Salmonella Isolates from Commercial Birds and Poultry Environment in Mississippi

Authors
item Pulido-Landinez, Martha -
item Washington, Paul -
item Thornton, Jay -
item Zhang, Yi -
item Sanchez-Ingunza, Roxana -
item Banda, Alejandro -
item Guard, Jean
item Pinheiro Do Nascimen, To, V -
item Magee, Danny -
item Mauel, Michael -

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 8, 2013
Publication Date: March 1, 2014
Citation: Pulido-Landinez, M., Washington, P., Thornton, J., Zhang, Y., Sanchez-Ingunza, R., Banda, A., Guard, J.Y., Pinheiro Do Nascimen, T., Magee, D., Mauel, M.J. 2014. Serotype and Antimicrobial Resistance Patterns of Salmonella Isolates from Commercial Birds and Poultry Environment in Mississippi. Avian Diseases. p. 64-70.

Interpretive Summary: Intergenic sequence ribotyping (ISR) analyzes single nucleotide polymorphisms occurring around a 5s ribosomal gene to assign serotype to Salmonella enterica isolates. The purpose of this research was to compare the results obtained by ISR with those obtained with the traditional Kauffman-White-Le Minor (KWL) scheme for the characterization and serotyping of Salmonella enterica isolates from commercial birds and poultry environment, previously isolated at the Poultry Research and Diagnostic Laboratory, Mississippi State University. ISR assigned serotype to 50 Salmonella enterica isolates showing an almost perfect agreement ('=89.58) between ISR and KWL (43 out of 48 isolates). The ISR method was also able to detect possible mixtures of serotypes in the samples. The antimicrobial resistance patterns (ARPs) of 12 serotypes of S. enterica (Enteritidis, Typhimurium, Kentucky, Bredeney, Mbandaka, Saintpaul, Montevideo, Cubana, Lille, Senftenberg, Johanesburg and one serotype UN0094) were determined using the minimum inhibitory concentration values. The antibiograms demonstrated differences between Salmonella serotypes and among isolates of the same serotype. All isolates were 100% susceptible to enrofloxacin and trimethoprim/sulfamethoxazole. The number of antimicrobials to which the isolates were resistant ranged from 2 to 9. Twenty two different ARPs were identified. ARP1 was the most frequently observed with resistance to two antimicrobials (spectinomycin and sulfadimethoxine). Forty isolates (80%) were resistant to 3 or more antimicrobials meeting the criteria to be designated as multidrug resistant.

Technical Abstract: To obtain information about Salmonella from commercial birds and poultry environments within Mississippi, 50 Salmonella enterica isolates were collected and characterized by Intergenic Sequence Ribotyping (ISR) serotyping and by determining antimicrobial resistance. ISR assigned serotype to all 50 Salmonella enterica isolates, whereas the Kauffman-White-LeMinor antibody-based schema assigned serotype to 48. Agreement between both methods was '=89.58. Within the set, 12 serotypes were detected. The antimicrobial resistance patterns of 12 serotypes, namely Enteritidis, Typhimurium, Kentucky, Bredeney, Mbandaka, Saintpaul, Montevideo, Cubana, Lille, Senftenberg, Johannesburg and one serotype UN0094, were determined using minimum inhibitory concentration values. The antibiograms demonstrated differences between Salmonella serotypes and among isolates of the same serotype. All isolates were 100% susceptible to enrofloxacin and trimethoprim/sulfamethoxazole. The number of antimicrobials to which the isolates were resistant ranged from 2 to 9. Twenty-two (22) different ARPs were identified, and ARP1, with resistance to spectinomycin and sulfadimethoxine, was most frequently observed. Forty isolates (80%) were resistant to 3 or more antimicrobials, and were thus designated multidrug resistant. Detection of a unique serotype and variation in antibiograms within the set demonstrates that it is important to periodically survey isolates from a region to follow epidemiological trends.

Last Modified: 10/23/2014
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