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United States Department of Agriculture

Agricultural Research Service

Research Project: Efficient Management and Use of Animal Manure to Protect Human Health and Environmental Quality

Location: Food Animal Environmental Systems Research Unit

Title: Robust, cross-laboratory validated PCR methods to track antibiotic resistance in agricultural settings

Authors
item Cook, Kimberly
item Moorman, Thomas
item Durso, Lisa

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 25, 2013
Publication Date: November 6, 2013
Citation: Cook, K.L., Moorman, T.B., Durso, L.M. 2013. Robust, cross-laboratory validated PCR methods to track antibiotic resistance in agricultural settings. ASA-CSSA-SSSA Annual Meeting Abstracts. Abstract.

Technical Abstract: In order to make informed decisions on how best to manage agricultural antibiotics, we need to be able to track and quantify the antibiotic resistant bacteria, and antibiotic resistance genes. PCR-based methods are widely used to both detect and quantify antibiotic resistance genes, however there are many technical issues associated with PCR development and operation that can impact the interpretation of the results. We propose a series of standards be routinely included in PCR assays designed to track and quantify antibiotic resistance in agriculturally associated and environmental samples, and recommend the cross-lab validation of both standard and quantitative PCR assays.

Last Modified: 12/21/2014
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