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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Wheat and Barley for Resistance to Biotic and Abiotic Stresses

Location: Wheat Genetics, Quality Physiology and Disease Research

Title: Identification and mapping of adult-plant stripe rust resistance in soft red winter wheat cultivar ‘USG 3555'

Authors
item Christopher, Mark -
item Liu, Shuyu -
item Hall, Marla -
item Marshall, David
item Fountain, Myron
item Johnson, Jerry -
item Milus, Eugene -
item Garland-Campbell, Kimberly
item Chen, Xianming
item Griffeyz, Carl -

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 15, 2012
Publication Date: January 20, 2013
Citation: Christopher, M.D., Liu, S., Hall, M.D., Marshall, D.S., Fountain, M.O., Johnson, J.W., Milus, E.A., Garland Campbell, K.A., Chen, X., Griffeyz, C.A. 2013. Identification and mapping of adult-plant stripe rust resistance in soft red winter wheat cultivar ‘USG 3555'. Plant Breeding. 132: 53–60. doi: 10.1111/pbr.12015.

Interpretive Summary: Stripe rust is an important disease of wheat worlwide. Adult plant resistance has been more durable than single gene resistance. Resistance to stripe rust was identified and characterized in a population from a cross between the resistant cultivar USG3555 and the susceptible cultivar Neuse. Genes conributing to resistance were discovered on chromosomes 1AS, 4BL, 7D from USG3555. Use of these markers and development of additional diagnostic markers will facilitate the incorporation and pyramiding of stripe rust resistance into soft red winter wheat lines via marker-assisted selection.

Technical Abstract: Little is known about the extent or diversity of resistance in soft red winter wheat (Triticum aestivum L.) to stripe rust, caused by the fungal pathogen Puccinia striiformis f.sp. tritici. The soft red winter (SRW) wheat cultivar ‘USG 3555’ has effective adult-plant resistance to stripe rust, which was characterized in a population derived from ‘USG 3555’/‘Neuse’. The mapping population consisted of 99 recombinant inbred lines, which were evaluated for stripe rust infection type (IT) and severity to race PST-100 in field trials in North Carolina in 2010 and 2011. Genome-wide molecular-marker screenings with 119 simple sequence repeats and 560 Diversity Arrays Technology (DArT) markers were employed to identify quantitative trait loci (QTL) for stripe rust resistance. QTL on chromosomes 1AS, 4BL and 7D of ‘USG 3555’ explained 12.8, 73.0 and 13.6% of the variation in stripe rust IT, and 13.5, 72.3 and 10.5% of the variation in stripe rust severity, respectively. Use of these and additional diagnostic markers for these QTL will facilitate the introgression of this source of stripe rust resistance into SRW wheat lines via marker-assisted selection.

Last Modified: 4/17/2014
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