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United States Department of Agriculture

Agricultural Research Service

Research Project: COTTON DISEASE MANAGEMENT STRATEGIES FOR SUSTAINABLE COTTON PRODUCTION

Location: Insect Control and Cotton Disease Research Unit

Title: Detoxification of the fusarium toxin fusaric acid by the soil fungus aspergillus tubingensis

Authors
item Crutcher, Frankie
item Liu, Jinggao
item Puckhaber, Lorraine
item Stipanovic, Robert
item Duke, Sara
item Bell, Alois
item Williams, Howard -
item Nichols, Robert -

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 27, 2013
Publication Date: January 27, 2014
Citation: Crutcher, F.K., Liu, J., Puckhaber, L.S., Stipanovic, R.D., Duke, S.E., Bell, A.A., Williams, H.J., Nichols, R. 2014. Detoxification of the fusarium toxin fusaric acid by the soil fungus aspergillus tubingensis. National Cotton Council Beltwide Cotton Conference. CD-ROM.

Technical Abstract: The fungal pathogen Fusarium oxysporum f. sp. vasinfectum (F.o.v.) causes cotton wilt and produces the toxin fusaric acid (FA). Previous research indicates that in the high producing strains of F.o.v., FA plays an important role in virulence. To address the problems of emerging virulent isolates such as Australian biotype and California race 4 isolates possessing high FA production capacity, mechanisms of FA detoxification in microorganisms have been evaluated. Screening of a soil sample from a cotton field infested with Reniform nematode and Fusarium identified and isolated an Aspergillus niger strain with high tolerance to FA. HPLC analysis of culture filtrates from A. niger grown in the presence of FA showed that FA degraded and decreased, and an unknown compound appeared and increased over time. Spectral analysis of the unknown compound and the chemical synthesis of the unknown compound identified the compound as 5-butylpyridine-2-methanol (fusarinol). The phytotoxicity of fusarinol compared to FA at different concentrations was measured by the ability of the compound to cause necrosis on cotton (Gossypium hirsutum L. cv. Coker 312) cotyledons. The toxic effects of fusarinol are significantly less than FA, providing a novel detoxification mechanism against FA mediated disease.

Last Modified: 8/21/2014
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