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United States Department of Agriculture

Agricultural Research Service

Research Project: Integrated Aquatic Animal Health Strategies

Location: Aquatic Animal Health Research

Title: Developing live bacterial vaccines by selecting resistance to antibacterials

Authors
item Wei Pridgeon, Yuping
item Klesius, Phillip

Submitted to: Global Aquaculture Advocate
Publication Type: Popular Publication
Publication Acceptance Date: April 12, 2014
Publication Date: May 1, 2014
Citation: Wei Pridgeon, Y., Klesius, P.H. 2014. Developing live bacterial vaccines by selecting resistance to antibacterials. Global Aquaculture Advocate. May/June:74-75.

Technical Abstract: Four chemicals were used in this study to modify bacterial isolates through chemical-resistance strategy. All bacteria were able to develop high resistance to gossypol. However, none of the gossypol-resistant isolate was attenuated. Although majority of the proflavine hemisulfate-resistant isolates were attenuated, all of them failed to provide significant protection to fish. Eight novobiocin- or ciprofloxacin- resistant Gram-positive bacteria were found to be attenuated. However, the protection they offered were less than 70%. Of all attenuated novobiocin- or ciprofloxacin- resistant Gram-negative isolates, only one was found to be relatively safe and highly efficacious. Our results suggest that the development of live attenuated bacterial vaccines that are safe and efficacious is challenging, although it is feasible.

Last Modified: 8/27/2014
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