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United States Department of Agriculture

Agricultural Research Service

Research Project: Identification and Analysis of Plant Architectural Genes in Maize

Location: Plant Gene Expression Center Albany_CA

Title: Unequal redundancies in KNOX genes

Authors
item Buldoc, Nathalie -
item Tyers, Randall -
item Freeling, Michael -
item Hake, Sarah

Submitted to: Plant Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 8, 2013
Publication Date: January 1, 2014
Citation: Buldoc, N., Tyers, R.G., Freeling, M., Hake, S.C. 2014. Unequal redundancies in KNOX genes. Plant Physiology. 164:229-38.

Interpretive Summary: The knotted1 (kn1) homeobox (knox) gene family was first identified through gain-of-function dominant mutants in maize. Class I knox members are expressed in meristems but excluded from leaves. In maize, a loss-of-function phenotype has only been characterized for kn1. Our analysis of kn1 and rs1 double mutants demonstrate an unequal redundancy between knox genes, with a role for rs1 only revealed in the complete absence of kn1.

Technical Abstract: To assess the function of another knox member, we characterized a loss-of-function mutation of rough sheath1 (rs1). rs1-mum1 has no phenotype alone but exacerbates several aspects of the kn1 phenotype. In permissive backgrounds in which kn1 mutants grow to maturity, loss of a single copy of rs1 enhances the tassel branch reduction phenotype while loss of both copies results in limited shoots. In less introgressed lines, double mutants can grow to maturity but are shorter. Using a KNOX antibody, we demonstrate that RS1 binds in vivo to some of the KN1 target genes, which could partially explain why KN1 binds many genes, but modulates few.

Last Modified: 11/23/2014
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