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United States Department of Agriculture

Agricultural Research Service

Title: The Development of a Homologous Radioimmunoassay for Chicken Insulin-Like Growth Factor-I

Authors
item McMurtry, John
item Francis Geoff L, - CSIRO DIV OF HUMAN NUTR
item Upton F Z, - CSIRO DIV OF HUMAN NUTR
item Rosselot Gaston, - UNIVERSITY OF CHILE
item Brocht, Donna

Submitted to: Poultry Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 23, 1994
Publication Date: N/A

Technical Abstract: Development of a homologous radioimmmunoassay (RIA) for chicken insulin-like growth factor-I (cIGF-I) and its use to investigate the developmental changes in IGF-I in the chicken and turkey is descibed. A double antibody RIA has been developed using recombinantly derived cIGF-I as antigen, radiolabelled tracer and standard. The resulting immunoassay has a minimum detection limit of 0.035 ng and effective dose of 2.5 ng. Dose response curves of chicken and turkey plasma and tissue extracts were parallel with cIGF-I standard. The antiserum is specific for IGF-I as no cross-reactivity with chicken IGF-II, insulin, glucagon, gastrin or avian pancreatic polypeptide was observed. We have also established that acid/ethanol extraction of chicken and turkey plasma reduced possible interference of IGF-binding proteins (IGFBPs) in the RIA. Comparison of IGF-I immunoactivity in unextracted and acid/ethanol-extracted samples following gel filtration under acidic and neutral conditions indicates that the cIGFBPs may be acid-labile. Analyses of samples from growing chickens and turkeys using the homologous avian reagents revealed higher IGF-I concentrations than if the IGF were quantified using heterologous mammalian-derived reagents. A similar pattern was observed when tissue extracts were assayed for IGF-I content. The application of the homologous RIA to monitor blood and tissue IGF-I levels during embryonic development and post-hatch growth in avian species will provide more accurate comparisons of results from studies on the role of IGF-I in growth and metabolism of domestic birds.

Last Modified: 7/31/2014
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