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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #315155

Title: Inhibition of porcine reproductive and respiratory syndrome virus in vitro by forsythoside A

Author
item YANG, MING - Beijing University Of Agriculture
item LU, YAN - Beijing University Of Agriculture
item MA, YUANYUAN - Peking University
item WU, GUOYING - Peking University
item Beier, Ross
item HUO, XIAOLIN - Beijing University Of Agriculture
item WU, GUOJUAN - Beijing University Of Agriculture

Submitted to: International Journal of Pharmacology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/21/2015
Publication Date: 3/6/2015
Publication URL: http://handle.nal.usda.gov/10113/60841
Citation: Yang, M., Lu, Y., Ma, Y., Wu, G., Beier, R.C., Huo, X., Wu, G. 2015. Inhibition of porcine reproductive and respiratory syndrome virus in vitro by forsythoside A. International Journal of Pharmacology. 4:394-399.

Interpretive Summary: Porcine Reproductive and Respiratory Syndrome (PRRS) is an important contagious disease of swine worldwide. The PRRS virus (PRRSV) is the causative agent of PRRS by airborne transmission and causes reproductive failures in breeding age swine resulting in mummification, still birth, late term abortion, and delayed return to estrus. Current control strategies against PRRSV are inadequate and there is a need for a new antiviral therapy against PRRSV. Pulmonary alveolar macrophages and MA104 derived monkey kidney MARC-145 cells can be used to replicate PRRSV infection. Forsythoside A is a glucopyranoside with a molecular weight of 624.59 derived from the fruit of Forsythia suspensa (Thunb.) Vahl. The effect of forsythoside A on the infection of Marc-145 cells by PRRSV was investigated in this study. It was found that forsythoside A had a significant protective effect on the pathological changes observed in Marc-145 cells as a result of PRRSV infection. The most protective effect was observed when forsythoside A treatment of Marc-145 cells were conducted prior to PRRSV infection. It was observed that forsythoside A inhibited PRRSV RNA synthesis and promoted secretion of IFN-a (a potent immune-defense protein) in infected Marc-145 cells. This research has established the preliminary data for further development of forsythoside A as an antiviral agent.

Technical Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) represents a significant challenge to the swine industry worldwide. Current control strategies against PRRSV are still inadequate and there is a need for new antiviral therapy method. Forsythoside is a compound derived from fruit of Forsythia suspensa (Thunb.). Vahl suspensa, a traditional antiviral Chinese medicine. The effect of forsythoside on the infection of Marc-145 cells by PRRSV was investigated through the in vitro antivirus action and cytopathic change assay. It was found that forsythoside had a significant protective effect on Marc-145 cells pathological changes, the protective activity from high to low was treatment prior infection, treatment of infected cells, direct virucidal effect, respectively. Furthermore, its effect on the PRRSV RNA capacity and secretion of IFN-a was also evaluated via measurement of the cytopathic changes, real time PCR and ELISA assay. It indicated that forsythoside A inhibited PRRSV RNA synthesis and promoted secretion of IFN-a, and in infected Marc-145 cells at a certain concentration. The research has prepared the ground for later development of forsythoside as an anti-virus agent.