ARS | Publication request: IN VITRO EXPOSURE OF CHICKEN IMMUNE CELLS TO 4,15-DIACETOXYSCIRPENOL RESULTS IN ALTERED IMMUNE FUNCTION

Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 1994
Publication Date: N/A

Technical Abstract: Sephadex-elicited broiler macrophages were exposed to 1, 12.5 and 25 ug/mL DAS in vitro. One h exposure resulted in dose-related reduction in cell viability, and increased cell damage in two replicate experiments. Uptake of opsonized SRBC was reduced by 25 ug DAS exposure (P < .05) whereas unopsonized SRBC uptake was reduced (P < .05) by both levels of DAS. Furthermore, incidence of Fc-R+ macrophages was reduced after exposure to both DAS concentrations. DAS (12.5 ug) suppressed Class I expression in bursacytes and splenocytes (mean fluorescence), but increased expression in thymocytes as determined by flow cytometry. Class II was suppressed in thymus, but increased in bursa and spleen cells. Class IV expression was not altered on any cell types examined. Antigen detected by K1 MCA (presumably adhesion molecule) was decreased in spleen, but no change was observed in thymic and bursal cells. These results suggest that DAS exposure may modulate cellular interactions and functions within the immune system.