Submitted to: Coccidiosis International Conference Proceedings
Publication Type: Research Notes
Publication Acceptance Date: November 1, 1994
Publication Date: N/A
Interpretive Summary: Avian coccidiosis causes severe intestinal damage and an estimated annual loss of $450 million to poultry industry. Vaccine for coccidiosis is not available. ARS scientists, in collaboration with Japanese scientists, developed a chicken monoclonal antibody to identify immunogenic proteins of coccidial parasites. The results suggest that chickens make antibodies to the antigens located at the anterior tip of the parasites. These studies may lead to the identification of novel coccidial vaccine antigens.
An ultrastructural study was carried out using immuno-electron microscopy to characterize the reactivity of a chicken monoclonal antibody. Recently, we have reported that this chicken monoclonal antibody, 6D-12-G10, recognized the antigens located at the apical complex of sporozoites by immunofluorescent microscopy. The monoclonal antibody, 6D-12-G10, showed a statistically significant inhibition of the sporozoite invasion of CD8+ T cells in vitro. In the immuno-electron microscopic study, the conoid of Eimeria acervulina sporozoites was identified with this chicken monoclonal antibody. Therefore these results show that the conoid antigens recognized by the monoclonal antibody, 6D-12-G10 may play an important role in the invasion of sporozoites into host cells.