ARS | Publication request: COMPARISON OF FOUR MOLECULAR MARKERS IN MEASURING RELATIONSHIPS AMONG SOLANUM SECTION ETUBEROSUM (SUBGENUS POTATOE)

Submitted to: American Journal of Potato Research
Publication Type: Abstract Only
Publication Acceptance Date: June 30, 1995
Publication Date: N/A

Technical Abstract: We evaluated chloroplast DNA (cpDNA), isozymes, single to low- copy nuclear DNA (RFLPs) and random amplified polymorphic DNAs (RAPDs) in terms of concordance and efficiency for systematic relationships of 15 accessions each of Solanum etuberosum and S. palustre, and four accessions of S. fernandezianum. These self-compatible, diploid (2n=24), and morphologically very similar taxa constitute all species in Solanum sect. Petota (the potato and its wild relatives). Genetic distance and multidimensional scaling results show general concordance of isozymes, RFLPs and RAPDs between all three taxa; cpDNA shows S. etuberosum and S. palustre to be more similar to each other than to S. fernandezianum. Interspecific sampling variance shows a gradation of resolution from allozyme (low) to RAPD to RFLP (high); while intraspecific comparisons graded from RFLPs (low) to RAPDs (high; lack of sufficient allozyme variability within species precluded comparisons for allozymes). Experimental error was low in RFLPs and RAPDs. These results leave unresolved the progenitor-derivative relationships within sect. Etuberosum.