Submitted to: Plant Molecular Biology Reporter
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 2, 1995
Publication Date: N/A
Interpretive Summary: Tomato spotted wilt virus (TSWV) is an important plant pathogen. The host range of TSWV includes more than 400 plant species. The wide host range of the virus and vector combined with the lack of effective management strategies leads to major worldwide economic losses. This article describes the replication of TSWV in the genetic model plant Arabidopsis thaliana. Ultimately, this work will provide novel sources of plant resistance to viral disease. The product of this work will be used by plant breeders to develop plant lines resistant to viruses.
Mechanical inoculation of Arabidopsis thaliana ecotype Columbia with tomato spotted wilt virus led to viral replication and spread as determined by dot blot and ELISA analysis. Severe symptoms were observed three to four weeks post-inoculation. Early symptoms were manifested as chlorotic spots on uninoculated leaves. Later in the infection process, some plants showed complete chlorosis and wilting prior to bolting. Bolts that were developed by infected plants were chlorotic and deformed. These preliminary results suggest that A. thaliana could become a model system for the genetic analysis of host factors required for the replication of viruses in the family Bunyaviridae, which includes viruses that cause important diseases of both plants and animals.