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United States Department of Agriculture

Agricultural Research Service

Title: Serologic Responses of Brucella Abortus Strain 19 Calfhood-Vaccinated Cattle Following Adult Vaccination with Strain Rb51

Authors
item Olsen, Steven
item Evans, D - VS APHIS
item Hennager, S - NVSL VS APHIS
item Cheville, Norman - IA STATE UNIV
item Stevens, Mark

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 14, 1995
Publication Date: N/A

Technical Abstract: This study was designed to determine if Brucella abortus strain RB51 (SRB51), which expresses small amounts of the lipopolysaccharide O-side chain, would cause anamnestic responses on serological surveillance tests when given to adult cattle that were calfhood vaccinated with B. abortus strain 19 (S19). Cattle vaccinated as adults with SRB51 that had been vaccinated in calfhood with S19 (n=40) had significantly greater (P<0.05) antibody titers against SRB51 at 4 and 8 weeks post- vaccination in the dot blot assay when compared to animals (n=10) not vaccinated with SRB51. At 4 and 8 wk following SRB51 vaccination, sera from all SRB51-vaccinates were negative on the card, buffered acid plate agglutination (BAPA), complement fixation (CF), standard tube agglutination (STA), and particle concentration fluorescence immunoassay (PCFIA) tests with the exception of 10 samples. Four animals were card positive at either 4 or 8 weeks following vaccination. The BAPA test identified 1 positive sample at 4 weeks and 5 positive samples at 8 weeks from SRB51-vaccinates that tested negative on the card test. This study indicates SRB51-vaccination of adult animals that were calfhood vaccinated with S19 may cause a small percentage of transient positive responses on brucellosis tests with high sensitivity, but will not cause detectable antibody titers when evaluated by other highly specific conventional serologic tests.

Last Modified: 8/29/2014
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