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United States Department of Agriculture

Agricultural Research Service

Title: Modification of a Technique for Support of Thin, Flexible Tissues for Microscopic Examination: Application to Turkey Air Sac Membranes

Author
item Kunkle, Robert

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 9, 1996
Publication Date: N/A

Interpretive Summary: Respiratory diseases are major causes of economic losses to the poultry industry. Lesions on the air sac membranes of birds are a principal cause of condemnations during slaughter examinations. Because of the delicate nature of these membranes, collection by the usual methods results in their total destruction or creation of artifacts so that microscopic analyses can nnot be done. An existing technique was modified for collection of avian air sac membranes and other thin, broad tissues to improve processing of samples for microscopy. The modified technique produced tissue sections free of confusing artifacts, and tissues maintained their natural orientation. Visualization of microscopic anatomic detail utilizing the technique was exceptionally good. This method can be used to study the microscopic anatomy of a variety of thin tissues, such as mesentery, intestine, diaphragm, veins, and arteries. The technique is applicable to evaluations of infectious disease, cancer, immune-mediated disease, and blood vessel derangement.

Technical Abstract: Modifications to the ring-stabilization technique for collection of avian air sac membranes were developed to allow dehydration and paraffin- embedding of samples to be done by an automated system and to simplify processing for transmission electron microscopy (TEM). The modified collection method utilized a pair of rings that were snapped together across an intervening membrane sample. The tissue and ring ensemble were embedded together. Paraffin-embedded tissues were collected with aluminum rings, while samples for TEM were collected with rings made of polymerized embedding medium. Tissue sections of excellent quality were obtained with the method. The technique is shown to be applicable to immunohistochemical studies.

Last Modified: 11/23/2014
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