ARS | Publication request: MOLECULAR MAPPING OF CARROT (DAUCUS CAROTA L.) USING RFLP AND RAPD MARKERS

Submitted to: Plant Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 8, 1996
Publication Date: N/A

Technical Abstract: Carrot is a cross-pollinated diploid with 2n=2x=18. It is biennial and any step towards early identification of useful genes would aid carrot breeding and genetics. The present study was initiated to develop a molecular map of carrot and identify markers linked to root- pigmentation genes and ergonomically useful characters like root diameter, root core diameter, root length and sugar type. For this purpose we are using FRS from a cross between a relative of B7262 (purple, P; yellow, Y2; low reducing sugar, Rs) and B9304 (non- purple, p; orange, y2, high reducing sugar, Rs). The FRS also segregate for multigenic traits including root diameter, root core diameter, and root length. 84 clones (representing about 17% of the clones evaluated to date) have shown RFLPs between the parents for at least one of the 4 enzymes (EcoR I, Hind III, Dra I and EcoR V). HindIII has shown the highest polymorphism among these enzymes and is thus being used for mapping the population with the above clones. Markers associated with P, Y2 and Rs as well as multigenic root traits are identified and RAPDs are included as we approach an 80-point linkage map of carrot. Details of the molecular map, as it further unfolds, will be discussed.