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ARS Home » Midwest Area » Columbia, Missouri » Biological Control of Insects Research » Research » Publications at this Location » Publication #67221
Title: NEWLY GENERATED CONTINUOUS CELL CULTURES AND CLONAL LINES FROM LEPIDOPTERA

Author
item Goodman, Cynthia
item McIntosh, Arthur

Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cell lines from a variety of insect species were generated, including the cloning of previously established cell lines. A total of 17 continuous cell cultures have been established from 7 species of insects (Lepidoptera), 3 insect stages (egg, larva, adult), and approximately 5 different tissue types (which primarily included ovarian, testes, fat body, ,and embryonic tissues). These cultures vary widely in their morphology an replication rates. Many of the cultures have been passaged more than 20 times, with all others having been subcultured at least twice. The newly established continuous cultures are presently being adapted to a commercial serum-free medium. These cultures are in the process of being subjected to biochemical analyses for identification purposes (including PCR). Three parental cell lines were used to generate clones, with numerous clones being generated from each. The clones were established either from parental lines growing in serum-free medium, or are presently being adapte to serum-free medium. The clones are also in the process of being characterized biochemically for identification purposes.