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Title: CONVERSION OF 12-HYDROXYOCTADECANOIC ACID TO 12,15-, 12,16- AND 12,17- DIHYDROXYOCTADECANOIC ACIDS WITH BACILLUS SP. U88

Author
item HUANG, J.-K. - WESTERN IL UNIV MACOMB IL
item KEUDELL, KENNETH - WESTERN IL UNIV MACOMB IL
item SEONG, SU-JIN - WESTERN IL UNIV MACOMB IL
item KLOPFENSTEIN, WILLIAM - WESTERN IL UNIV MACOMB IL
item WEN, LISA - WESTERN IL UNIV MACOMB IL
item Bagby, Marvin
item NORTON, ROBERT - 3620-30-00
item VESONDER, RONALD - 3620-30-00

Submitted to: Biotechnology Letters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/26/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary: During a program to discover organisms that will modify soybean and other vegetable oils to give new value added products, a new Bacillus strain was found. This organism readily converts a fatty acid obtained from castor oil to fatty acids containing an additional hydroxyl group on either carbon atom 15, 16 or 17 of the 18 carbon fatty acid. Hydroxy fatty acids are used in lubricants, greases, cosmetics and other commercial products.

Technical Abstract: A new microbial isolate, Bacillus sp. U88, transformed 12-hydroxyoctadecanoic acid to 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids when grown aerobically in 1% yeast extract medium at 30C and shaken at 450 rpm for 5 days. These three dihydroxyfatty acids were derivatized with trimethylsilyl ether and identified by GC-MS analysis. The yield of 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids after 5 day incubation were 2.8%, 7.1% and 1.1%, respectively.