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ARS Home » Research » Publications at this Location » Publication #68601
Title: SOMATOTROPH FUNCTION IN THE NEONATAL PERIOD OF THE PIG

Author
item Matteri, Robert
item CARROLL, JEFFERY - TEXAS A&M UNIVERSITY
item Klir, John

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 7/26/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The objective of this study was to evaluate developmental changes in in vitro somatotroph function in neonatal pigs. Eight male piglets were sacrificed at 1, 7, 14, 21, 28, 35, and 42 days of age. Serum concentrations of GH and IGF-1 were determined. Pituitary cells from each pig were cultured for 3 days, and then stimulated for 3 hr with .1, 1, or 10 nM GHRH; 2 mM 8-Br-cAMP; or 100 nM phorbol myristate acetate. Mean serum and intracellular (ic) hormone values are expressed as ng/ml serum or ng/250,000 cells in the table below. --------------- Age (Days) --------------- 1 7 14 21 28 35 42 SEM P= GH 31.2 5.64 4.15 7.27 14.5 9.79 8.44 2.72 .001 icGH 695 636 589 417 370 512 530 65.3 .011 IGF1 32.3 137 128 76.8 59.5 68.5 174 16.9 .001 Wt(kg 1.46 3.19 5.58 6.49 6.89 9.85 13.0 0.58 .001 In vitro GH secretion was significantly stimulated by secretagogue treatments (p < .001). Age-related changes in in vitro GH secretion (p < .001) were consistent with changes in icGH. An Age X Secretagogue interaction (p < .001) was detected which reflected a higher sensitivity to stimulation in cells from the younger animals. For example; GH concentrations (ng/ml) in culture following treatment with 0, .1, 1, or 10 nM GHRH, cAMP, and PMA were 273.3, 433.7, 669.1, 705.4, 884.6, and 2111.4 in the 1-day-old group and 150.7, 248.7, 324.7, 354.2, 276.4, and 674.6 in the 28-day-old group, respectively. These results demonstrate relatively rapid changes in somatotroph hormone content and secretory responses to stimulation during the neonatal period of the pig.