Author
Ahlgren, Jeffrey | |
Cote, Gregory | |
Leathers, Timothy |
Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only Publication Acceptance Date: 8/4/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Leuconostoc mesenteroides B-21297 is a mutant of L. mesenteroides B-1355 that is essentially free of dextransucrase activity and produces a two-fold increase in the alternansucrase activity. The purification of the extracellular alternansucrase activity from B-21297 incorporated the use of a novel alternanase enzyme to partially degrade endogenous alternan, which significantly enhanced the purification of the alternansucrase by ion exchange chromatography. Electrophoretic analysis of the purified enzyme showed a series of enzymatically active bands that range from 135 to 145 kDa in molecular weight. Preparative electrophoresis was used to prepare individual protein bands for protein microsequencing analysis. |