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United States Department of Agriculture

Agricultural Research Service

Title: Use of Monoclonal Antibodies to Detect Microorganisms in An Anaerobic Continuous Culture System That Is Protective in Chickens Against Salmonellatyphimurium

Authors
item Young, Colin
item Durant, Juliette - TEXAS A&M UNIVERSITY
item Ricke, Steven - TEXAS A&M UNIVERSITY
item STANKER, LARRY
item NISBET, DAVID
item Deloach, John

Submitted to: World Congress on Anaerobic Bacteria and Infections
Publication Type: Abstract Only
Publication Acceptance Date: October 10, 1995
Publication Date: N/A

Technical Abstract: Intestinal bacteria from adult chickens have been successfully grown under anaerobic continuous culture in chemostats. We have previously described such an anaerobic culture system (CF3) containing some 29 bacterial species. The administration of CF3 cultures to newly hatched chicks results in exclusion of Salmonellae and is known as competitive exclusion (CE). In order to determine the protective capacity, and/or fate of these individual bacteria in the bird we have generated a panel of monoclonal antibodies. Monoclonal antibodies have been produced against the following bacterial species in CF3; Veillonella spp, Eubacteria spp, Fusobacteria spp, and Proprionobacteria spp as well as against other anaerobic microorganisms. Data is presented on the specificity, isotype (IgA, IgM, IgG1, IgG2a,IgG2b, and IgG3) and sensitivity (10**2-10**4 cfu) of each of these monoclonal antibodies. Furthermore these monoclonal antibodies have been used to enumerate specific bacteria in the CF3 culture and in the ceca of chicks inoculated with CF3.

Last Modified: 7/28/2014
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