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United States Department of Agriculture

Agricultural Research Service

Title: Application of Molecularly-Imprinted Polymers for Rapid Sample Cleanup: Immunochemical and Hplc Analysis

item Muldoon, Mark
item Stanker, Larry

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 1995
Publication Date: N/A

Technical Abstract: Molecular imprinting technology has many potential applications in residue analysis. This technique involves the synthesis of polymers in the presence of an imprint molecule (analyte). After extracting the imprint molecule, the polymer can then be used as a specific binding matrix for sample analysis. We have investigated the use of molecularly-imprinted polymers as solid phase matrices for extraction and cleanup of biological sample extracts. An anti-atrazine polymer was used to cleanup organic extracts of beef liver and fat. Recovery of atrazine spiked at 1.0 and 0.1 ppm in beef liver was 109.7 and 81.0%, respectively, in the crude extracts as determined by HPLC. Following solid phase extraction, recoveries were 102.7 and 67.0%. Analysis of the crude extract from the 0.1 ppm atrazine spiked liver by ELISA gave a recovery of 5.1%. However following solid phase extraction, the recovery was 65.5%. Crude tissue sample extracts greatly interfered with the ELISA. However, the use of molecularly-imprinted solid phase extraction (MISPE) allowed for the rapid analysis of complex biological matrices using immunoassay.

Last Modified: 4/22/2015
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