Author
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Guerrero, Felicito |
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Submitted to: Peptides
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 10/19/1996 Publication Date: N/A Citation: N/A Interpretive Summary: STKR is a 4118 bp clone from a stable fly, Stomoxys calcitrans, cDNA library which encodes a protein with significant amino acid similarity to tachykinin-like peptide receptors in other organisms. Tachykinins and their receptors are involved in neuromuscular and sensory functions in both vertebrates and invertebrates. PCR was utilized to examine the receptor gene expression from various life stages of the stable fly and determine i which stage the mRNA was most abundant. STKR expression was significant in pupae and adults, particularly in isolated adult fly gut. Expression in development embryos was low but detectable, while larval expression was intermediate between early embryos and pupae. Technical Abstract: STKR is a 4118 bp clone from a stable fly, Stomoxys calcitrans, cDNA library which encodes a protein with significant amino acid identity to tachykinin-like peptide receptors. RT-PCR was utilized to examine the transcriptional expression from various life stages of the stable fly. STKR expression was significant in pupae and adults, particularly in isolated adult fly gut. Expression in developing embryos was low but detectable, while larval expression was intermediate between early embryos and pupae. |
