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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Transformation of Kenaf

Authors
item Srivatanakul, Metinee - TEXAS A&M-COLLEGE STATION
item Smith, Roberta - TEXAS A&M-COLLEGE STATION
item Sij, John - TEXAS A&M-BEAUMONT
item Webber, Charles

Submitted to: Fifth Chemical Congress of North America
Publication Type: Abstract Only
Publication Acceptance Date: November 11, 1997
Publication Date: N/A

Technical Abstract: A practical genetic transformation method for kenaf requires that the system is rapid genotype independent, and results in high frequency of transformants. A system is described which uses an easily regenerated explant, the 3-to-4-day-old shoot apex, and Agrobacterium tumefaciens as the gene vector. Shoot apices were isolated, surface sterilized, and germinated on a Murashige and Skoog (MS) salts medium. Shoot apices were co-cultivated with different Agrobacterium tumefaciens strains, LBA4404, EHA101S and Z707S, containing genes for resistance to phosphinotricin (ppt) and hygromycin (hyg). After co-cultivation for 3 days in the light, shoot apices were transferred to the selective medium containing 10 mg/l ppt or 16 mg/l hyg formed roots on MS medium. The highest percentages of rooted plants on selective medium were obtained from shoots that were co-cultivated with Agrobacterium strain LBA4404.

Last Modified: 4/20/2014
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