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United States Department of Agriculture

Agricultural Research Service

Title: Production of Gamma-Interferon by Peripheral Blood Mononuclear Cells: An Important Diagnostic Tool for Detection of Subclinical Paratuberculosis

item Stabel, Judith

Submitted to: International Colloquium on Paratuberculosis
Publication Type: Proceedings
Publication Acceptance Date: October 4, 1996
Publication Date: N/A

Technical Abstract: Peripheral blood mononuclear cells were isolated from noninfected control cows and from cows with either subclinical or clinical paratuberculosis (Johne's disease). Cells were incubated for 6, 12, 24, and 48 hours in complete medium with the following mitogens: concanavalin A (ConA), phytohemagglutinin-P (PHAP), pokeweed mitogen (PWM), and E. coli lipopolysaccharide (LPS). In addition, cells were incubated for the same time periods with a Mycobacterium paratuberculosis sonicate (MpS) and live and heat-killed M. paratuberculosis at 10:1 bacteria to cell ratio. After incubation, cell-free supernatants were analyzed for gamma-interferon (gamma-IFN) activity. Cells from subclinical cows produced significantly with mitogens, ConA, PHAP, and PWM. Levels of gamma-IFN were produced by cells isolated from subclinical animals compared to cells from clinical cows and noninfected controls. Stimulation of cells with heat-killed or live M. paratuberculosis evoked a similar response. This study indicates that gamma-IFN production by peripheral blood mononuclear cells in response to M. paratuberculosis antigen may be an important diagnostic tool for the detection of paratuberculosis in subclinical animals.

Last Modified: 4/19/2015
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