|Sasai, K - OSAKA PREFECTURE UNIV|
|Yoshimura, K - OSAKA PREFECTURE UNIV|
|Withanage, G. S. - OSAKA PREFECTURE UNIV|
|Fukata, T - OSAKA PREFECTURE UNIV|
|Baba, E - OSAKA PREFECTURE UNIV|
|Arakawa, A - OSAKA PREFECTURE UNIV|
Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 1, 1997
Publication Date: N/A
Interpretive Summary: The incidence of Salmonellosis in chickens is increasing worldwide and recombinant vaccine for salmonellosis is not available. To develop an immunological control strategy, understanding the host cellular response is crucial. In this paper, scientists at the University of Osaka collaborated with an ARS scientist to investigate the lymphocyte changes during salmonellosis. The results indicate that a significant change in lymphocyte subpopulations occurs after salmonella colonization. This finding will lead to a better understanding of host immunity to salmonella.
Technical Abstract: The percent changes in the lymphocyte subpopulations expressing CD3, CD4, CD8, pan lymphocyte, IgA, IgG and IgM cell surface antigens were assessed in the spleen and thymus of chickens following the infection with Salmonella enteritidis using flow cytometric analysis. The percentages of IgA+ and IgM+ cells in the spleen increased significantly at 6 days post primary S. enteritidis inoculation, whereas the percentage of CD3+ lymphocytes in the thymus decreased. At 2 and 4 days after challenge infection with S. enteritidis, the percentage of IgA+ cells in the spleen of the challenged group was significantly higher than the uninoculated and immunized groups. In the thymus, the percentages of CD8+ cells in the challenged group and immunized-challenged group increased significantly as compared with the uninoculated group and immunized group on day 2 post challenge infection. Also the percentage of CD4+ cells in the spleen was signficantly enhanced at 2 days post challenge infection in the immunized and challenged chickens. However, other cell populations showed minimal changes after primary and challenge infections. These results indicate that the S. enteritidis infection induces a significant change in lymphocytes.