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United States Department of Agriculture

Agricultural Research Service

Title: Use of Ribotyping to Distinguish Isolates of Bordetella Bronchiseptica - for the 1997 Annual Conf. of the Assoc. of Vet. Teachers and Research Workers, Scarborough, Uk, March 24-27, 1997

item Register, Karen
item Boisvert, Agatha - IA STATE UNIV., AMES, IA
item Magyar, Tibor - VET. MED. RES. INST., HU

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 28, 1997
Publication Date: N/A

Technical Abstract: Bordetella bronchiseptica is a common pathogen of the upper respiratory tract in a large number of mammalian species. Cross-species transmission has been proposed as one mechanism of spread. However, there are few epidemiologic studies addressing the frequency of cross-species transmission, largely due to the absence of a simple and reliable typing system. In this study, a total of 123 B. bronchiseptica strains, isolated from 11 different host species worldwide, were characterized by ribotyping with the restriction enzyme PvuII. Seventeen distinct ribotypes were identified, each of which contained 5 to 7 restriction fragments ranging in size from 1.8 Kb to 5.6 Kb. Approximately 90% of swine isolates were identified as ribotype 3. Isolates from dogs also displayed little variation; 74.1% were found to be ribotype 4. Strains obtained from the remaining 9 host species were represented by 16 different ribotypes. There ewas no apparent association between geographic location and ribotype. Thi technique may be useful for epidemiologic studies investigating the transmission of B. bronchiseptica, both within and among species.

Last Modified: 4/22/2015
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