|Evermann, J - WASHINGTON STATE UNIV|
|Landgraf, J - USDA-APHIS-NVSL-DVL|
|Frey, M - USDA-APHIS-NVSL-DVL|
Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Proceedings
Publication Acceptance Date: May 2, 1997
Publication Date: N/A
Technical Abstract: To examine the relationship between ruminant pestiviruses isolated in the U.S. and Canada, we surveyed 326 pestiviruses isolated from cattle, goats, sheep, and llamas. Based on differential polymerase chain reaction (PCR) amplification of sequences from the 5' untranslated region, 160 of the 326 viruses were characterized as bovine viral diarrhea virus (BVDV) 1 isolates sand 152 were characterized as BVDV 2. Of the original 326 pestiviruses, 1 viruses could not be amplified using PCR primers specific for BVDV isolates. All 14 of these pestiviruses were isolated from small ruminants. Phylogenetic analysis, based on sequences from the 5' UTR, segregated these 14 viruses into a genotype separate from BVDV 1, BVDV 2, and hog cholera virus. The virus BD31, available from the American Type Culture Collection as a border disease-type virus (BDV), was also segregated into this genotype. Viruses from this BD31-like genotype (BDV genotype) could be differentiated from viruses from other pestivirus genotypes by virus neutralization using polyclonal sera and by binding of monoclonal antibodies.