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United States Department of Agriculture

Agricultural Research Service

Title: Detection and Identification of Clavibacter Xyli Subsp. Xyli Using Pcr with a Pair of Highly Specific Primers.

item Pan, Yong-Bao
item Grisham, Michael
item Burner, David
item Damann, K - DEP PLANT PATH; LSU
item Wei, Q - OICD

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: August 4, 1997
Publication Date: N/A

Technical Abstract: The objective of this study was to develop a PCR protocol for the detection xyli subsp. xyli (Cxx). Clavibacter xyli subsp. xyli bacteria were isolate sugarcane tissue. Generic PCR with universal primers L1/G1 on Cxx genomic amplified a 566 bp product from the 16S and 23S ribosomal intergenic transc (ITS) region. The 566 bp product was cloned into a plasmid vector and sequ uNCBI BLASTN program was used to search for non-redundant GenBANK+EMBL+DDBJ +PDB database sequences that were highly homologous to the Cxx sequence. U programs for multiple sequence alignment and primer design, we developed tw PCR primers. A PCR protocol with these two primers amplified an expected C 438 bp product from genomic DNA samples of 21 Cxx strains collected worldwi cultured Cxx cells, and from Cxx-infected sugarcane tissue. No amplificati other bacterial species was observed. Extraction of Cxx genomic DNA was no prior to PCR assays.

Last Modified: 4/22/2015
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