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United States Department of Agriculture

Agricultural Research Service

Title: Effects of Recombinant Bovine Granulocyte-Macrophage-Colony Stimulatory Factor Administration on the Bovine Immune System and Resistance to Intramammary Challenge with Escherichia Coli

item Kehrli Jr, Marcus
item Shuster, Dale - AMERICAN CYANAMID, NJ
item Stabel, Judith
item Stevens, Mark - FORMER USDA, ARS, NADC
item Thurston, John - RETIRED USDA, ARS, NADC
item Daley, Michael - AMERICAN CYANAMID, NJ

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: April 20, 1997
Publication Date: N/A

Technical Abstract: An experiment was conducted to determine the immunomodulatory activity of recombinant bovine granulocyte-macrophage colony stimulatory factor (rbGM-CSF) in lactating dairy cows. Administration of rbGM-CSF was via single daily subcutaneous injection on alternating sides of the lateral cervical region at 0, 0.5, or 2.5 micrograms/kg body weight for eight consecutive days. Control cows received a placebo saline injection. Cows were challenged in one mammary quarter with 41 colony forming units of Escherichia coli after receiving seven daily injections of rbGM-CSF to measure any effects on experimentally-induced mastitis. A dose-dependent febrile response developed upon repeated administration of rbGM-CSF. Increases in rectal temperatures in response to rbGM-CSF administration after the first day averaged 0.37 degrees C greater than control cows within 5 to 6 h. By 9 to 10 h after rbGM-CSF injection, increases in rectal temperatures averaged 0.59 degrees C over control cows. Rectal temperatures returned to control levels by 24 h after each injection. Associated with the febrile responses were increases in respiration and heart rates.

Last Modified: 4/18/2015
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