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United States Department of Agriculture

Agricultural Research Service

Title: Prospects for Use of Glyptapanteles Indiensis Polydnavirus DNA for Stable Insect Cell Transformation

Authors
item Gundersen-Rindal, Dawn
item Lynn, Dwight
item Mckelvey, Terry - US PATENT OF. CRYSATL CY
item Taylor, Philip
item Dougherty, Edward

Submitted to: International Workshop on Transgenesis of Invertebrate Organisms
Publication Type: Abstract Only
Publication Acceptance Date: May 9, 1997
Publication Date: N/A

Technical Abstract: The parasitic wasp Glyptapanteles indiensis infects its host, Lymantria dispar (gypsy moth) with polydnavirus(GiPDV)during parasitization. Recently it was shown that a portion of the GiPDV viral genome can persist in several lepidopteran cell lines of somatic origin, stably integrated into the chromosome of the cells (McKelvey et al., 1996). The apparent ability of GiPDV DNA to integrate is of interest for its use as a vector. It appears that one GiPDV segment of the 10 or more comprising the GiPDV genome is integrated into L. dispar cells that have been infected with GiPDV. This GiPDV segment has been cloned and evaluated for its genetic organization. The integration border at the L. dispar cellular and GiPDV viral junction has been isolated and the border sequences determined. Of interest for GiPDV DNA as a vector is its apparent ability to transform cell lines from insects of agricultural importance. The range of species into which GiPDV DNA could integrate was evaluated for several insect cell lines. DNAs from somatic insect cell lines infected with GiPDV and from non-infected insect cell lines were evaluated for GiPDV sequences. GiPDV DNA fragments were detected in GiPDV transformed but not in non-transformed cell line DNAs. These results were confirmed by PCR. A GiPDV DNA-derived vector has utility for transformation of somatic tissue and potential for transgenesis of germline of many insect species.

Last Modified: 9/2/2014
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