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United States Department of Agriculture

Agricultural Research Service

Title: Differential Arrestment of Apolysis and Ecdysis in Larvae of Trichoplusia Ni Treated with the Venom from the Ectoparasitoid Wasp Euplectrus Comstockii (Family: Eulophidae)

item Wright, Maureen - UNIV OF MISSOURI
item Coudron, Thomas
item Brandt, Sandra - UNIV OF MISSOURI

Submitted to: International Entomophagous Insect Workshop
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 1997
Publication Date: N/A

Technical Abstract: The wasp Euplectrus comstockii injects a venom into the hemocoel of its lepidopteran hosts that arrests larval-larval apolysis and ecdysis. In previous studies a 66kDa protein that had arrestment activity was isolated from the venom. In the present studies hemolymph taken from 3rd instar Trichoplusia ni larvae 0-4 h post-parasitism did not arrest apolysis, but did cause arrestment of ecdysis when injected into 4th instar larvae. Larvae injected with hemolymph from insects 24 h post-parasitism developed normally. Antibodies to both total venom and to the N-terminus of the ecdysis-arresting protein were used to follow the fate of venom proteins within the host. Loss of arrestment activity was correlated with the disappearance of the ecdysis-arresting protein. Effect of venom titer, host factors, and proteases on the quality and extent of arrestment activity were determined.

Last Modified: 4/21/2015
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