Submitted to: Keystone Symposia on Molecular and Cellular Biology
Publication Type: Proceedings
Publication Acceptance Date: March 2, 1997
Publication Date: N/A
Technical Abstract: A partially recessive locus (mh) causes a heritable syndrome of generalized muscular hypertrophy in cattle, a combined result of both cellular hyperplasia and hypertrophy. Belgian Blue cattle, homozygous at mh, display markedly increased muscle mass and lean:fat ratio. Limb bone density, organ size, and reproduction are also affected. Since mh maps to BTA2, we constructed an integrated physical and genetic linkage map of seven genes in common between BTA2 and HSA2 to identify possible candidate cattle genes from comparative analysis. Our data demonstrate that overall syntenic conservation is maintained between BTA2q12-q42 and HSA2q13-q32, encompassing the genes ALPI, NRAMP, TNP1, NEB, GCG, COL3AI, and PROC. However, complex rearrangement of some genomic segments during evolution has occurred, as the gene order within the syntenic groups are significantly different in cattle than in humans. The data suggest that the human equivalent of the bovine mh locus lies close to the gene for Protein C (PROC) on the human map. An extensive mapping study is underway to determine the breakpoints of conserved synteny on either side of PROC and to more accurately determine on which side of PROC the mh locus may lie.