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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Characterization of Wheat-Wheatgrass Group 7 Translocation Lines and Localization of Barley Yellow Dwarf Virus Resistance Gene(s) in Wheat (Triticum Aestivum L.)

item Francki, Michael - PURDUE UNIVERSITY
item Crasta, Oswald - PURDUE UNIVERSITY
item Bucholtz, Dennis
item Sharma, Hari - PURDUE UNIVERSITY
item Anderson, Joseph

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 19, 1997
Publication Date: N/A

Technical Abstract: Barley Yellow Dwarf Virus (BYDV) is the most significant viral pathogen in wheat and other cereals world-wide. Because, wheat cultivars lack resistance to BYDV the intermediate wheatgrass Thinopyrum intermedium, an excellent source of resistance to BYDV, has been exploited in the soft red winter wheat breeding program at Purdue University, USA. A group 7 chromosome from Th. intermedium containing BYDV resistance was substituted for wheat chromosome 7D by intergeneric hybridization. This germplasm was used as parental material in further breeding strategies to restore much of the wheat genetic background but retaining BYDV resistance. A strategy involving backcrossing the group 7 disomic alien substitution line (P29), irradiating and selfing produced a series of resistant translocation lines with reduced amounts of alien chromatin. A preliminary molecular study using homoeologous group 7 RFLP markers has shown that the alien chromosome eresponsible for BYDV resistance in P29 is syntenic to wheat group 7A and 7 chromosomes. Using this information, several resistant translocation lines were identified having less than half the long arm of the alien group 7 chromosome. Additional studies were done to further localize resistance genes on the long arm of the alien chromosome. Analysis of susceptible translocation lines using homoeologous group 7 markers have localized BYDV resistance gene(s) on the distal end of the long arm of the alien chromosome. Although these susceptible lines are not beneficial for breeding purposes, they are extremely useful for physical mapping of BYDV resistance.

Last Modified: 4/22/2015
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