Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 1997
Publication Date: N/A
Technical Abstract: The purpose of this study was to determine whether Bordetella bronchiseptica was able to adhere, enter, and survive in porcine alveolar macrophages as well as to determine the effects the bacteria have on the macrophages. Modulatable B. bronchiseptica isolated from a pig with atrophic rhinitis (KM22) grown at 37 C (bvg+) or 23 C (bvg-) and a non- modulatable mutant strain (bvg-) (MBA4) were added to cultures of alveolar macrophages flushed from the lungs of conventional pigs. Fluorescence staining was used which differentiates bacteria adhered to the outside of cells from internalized bacteria. KM22 grown at 23 C (bvg-) adhered to the macrophages, and was internalized, in greater numbers than was the KM22 grown at 37 C (bvg+). MBA4 (bvg-) adhered minimally to the surface of the macrophages but was internalized in numbers comparable to those of the KM22 propagated at 23 C (bvg-). The addition of B. bronchiseptica antiserum decreased adherence to cells and did not appear to opsonize or increase th number of bacteria seen intracellularly. Ongoing studies investigating the viability of the intracellular bacteria and macrophages, as well as the functional attributes of the macrophages, will be presented.