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United States Department of Agriculture

Agricultural Research Service

Title: Hplc and Immunoassay Detection of the Coccidiostat Halofuginone in Chicken Liver Samples Obtained from Commercial Slaughter Houses

item Beier, Ross
item Stanker, Larry

Submitted to: Analytical Chemist
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 1998
Publication Date: N/A

Technical Abstract: The quinazolinone, halofuginone (Hal), is a feed additive used world wide to prevent coccidiosis in commercial poultry (chickens and turkeys) production. The current regulatory method utilizes a long digest and chemical extraction procedure followed by HPLC analysis. A cELISA screening procedure would drastically improve sample throughput, decrease the cost per sample, decrease the time required to run a sample, and decrease organic solvent wastes. A quick and economical competitive enzyme-linked immunosorbent assay (cELISA) method for determining Hal residues in chicken liver tissue has been developed, and evaluated using spiked and incurred samples. In addition, a cELISA-HPLC combined large scale sample analysis from slaughter houses was conducted during 1997 to correlate the cELISA method with the existing HPLC analysis method. A total of 482 samples were analyzed by both the cELISA method and the HPLC method. This final data set will be presented and evaluated.

Last Modified: 4/22/2015
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