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ARS Home » Research » Publications at this Location » Publication #88627
Title: BIOCONVERSION OF OLEIC ACID TO A SERIES OF KETO-HYDROXY AND DIHYDROXY ACIDSBY BACILLUS STRAIN NRRL BD-447

Author
item Lanser, Alan
item Manthey, Linda
item BERRY, KATHERINE - ARS/USDA/NCAUR, PEORIA,IL
item BENE, HENRY - ARS/USDA/NCAUR, PEORIA,IL

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/13/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Fifty-eight strains of eight Bacillus species were selected from the ARS Culture Collection to investigate their ability to bioconvert oleic acid to unique hydroxy or keto derivatives. Addition of 0.5% oleic acid substrate to a whole-cell culture of Bacillus strain NRRL BD-447 produced mixtures of mono-, di- and tri-substituted compounds. These products appear to contain combinations of keto- and hydroxy- groups. One of the major products, 7-hydroxy-16- keto-9-octadecenoic acid, was isolated by HPLC and its structure determined by GC-MS and NMR. Isolation and identification of other major products is described.