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United States Department of Agriculture

Agricultural Research Service

Title: Western Blot Analyses of Chicken Monoclonal Antibodies to Various Species of Avian Coccidia

item Sheriff, Rahmat - VRI, MALAYSIA
item Sasai, K - VRI, MALAYSIA
item Lillehoj, Hyun
item Matsuda, H - HIROSHIMA UNIV
item Fukata, T - OSAKA PREF UNIV
item Miyamoto, T - OSAKA PREF UNIV
item Baba, E - OSAKA PREF UNIV

Submitted to: Malaysian Society of Parasitology and Tropical Medicine
Publication Type: Abstract Only
Publication Acceptance Date: July 30, 1998
Publication Date: N/A

Technical Abstract: Species-specificity and cross-reactivity of chicken monoclonal antibodies (mAbs) developed against Eimeria acervulina (EA) sporozoites were determined by Western blotting analysis using the sporozoites of seven different avian Eimeria species such as EA, E. brunetti (EB), E. maxima (EM), E. mitis, E. necatrix (EN), E. praecox (EP) and E. tenella (ET). Five different mAbs showed similar reactivity on the apical complex of the sporozoite. However, in this study, using sonicated sporozoites of the seven Eimeria species as antigen, all mAbs (except one) recognized multiple bands. One of the mAbs, 8E-1 recognized multiple bands in all Eimeria species that were examined. MAbs HE-4 recognized the multiple bands of EA, EM, EN and ET; 8D-2 recognized the multiple bands of EA, EN and ET; 5D-11 recognized the multiple bands of EP, EA, EM, EB, EN and ET. One mAb, 8C-3, recognized only one band of EA. The predominant bands of mAbs had molecular weights of approximately 15, 32, 45 and 260 kilodaltons.

Last Modified: 4/18/2015
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