CELLULAR IMMUNE RESPONSES OF PIGS TO PARENTERAL VACCINATION WITH A SERPULINA HYODYSENTERIAE WHOLE CELL SONICATE OR PEPSIN-DIGEST BACTERIN

Authors: WATERS, W - IA STATE UNIV., AMES, IA; Sacco, Randy; DORN, A - IA STATE UNIV., AMES, IA; PENLAND, V - IA STATE UNIV., AMES, IA; KHALIFEH, M - IA STATE UNIV., AMES, IA; WANNEMUEHLER, M - IA STATE UNIV., AMES, IA

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/28/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The specific objective of this study was to compare cellular immune responses of pigs vaccinated with differing adjuvant and antigenic preparations of Serpulina hyodysenteriae. Pigs received three doses of either a pepsin-digest or whole cell sonicate preparation of S. hyodysenteriae in combination with either squalene or incomplete Freund's adjuvant. Control pigs received adjuvant alone. Upon in vitro antigen stimulation, peripheral blood lymphocytes from vaccinated pigs produced significant quantities of IFN-gamma as compared to lymphocytes from control pigs. In addition, these responses were diminished by either depletion of CD4 expressing cells or treatment with pIL-10. Vaccinated pigs also had significant antigen-specific lymphocyte proliferative responses in comparison to control pigs. A significant proportion of CD3+ lymphocytes proliferated in response to spirochetal antigen. Of the individual CD3+ subpopulations, the CD4+/CD8+ cells demonstrated the greatest degree of proliferation (73% of total). In addition to in vitro cell-mediated responses, vaccinated pigs demonstrated antigen-specific delayed type hypersensitive (DTH) responses. However, no significant differences were detected between antigen preparations or adjuvant usuage in either IFN-gamm duction, proliferation, or DTH responses. These data show that parenteral vaccination with a S. hyodysenteriae bacterin induces a measurable peripheral blood cellular immune response.