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United States Department of Agriculture

Agricultural Research Service

Title: Mapping Chicken Genes Using Preferential Amplification of Specific Alleles

Authors
item Smith, Eugene - RETIRED-ARS
item Cheng, Hans

Submitted to: Microbial and Comparative Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 1, 1998
Publication Date: N/A

Interpretive Summary: Genetic maps are comprised of highly informative DNA markers and genes for the basis of modern molecular genetics. Armed with these powerful tools, it is possible to identify agriculturally important genes. However, this is a difficult process and to circumvent some of the problems, many people are taking advantage of the high degree of conservation between their organism of interest, and humans or mouse. To make these comparative maps, genes are mapped in both species. This paper describes a novel technique to map genes using the chicken as an example. Advances like these will hasten the progress of gene identification and ultimately, the consumer as superior animals will be more easily bred.

Technical Abstract: To map the chicken genome, an international reference population was developed at our laboratory (East Lansing, MI) using an F2 backcross between inbred jungle fowl (JF) and inbred white leghorns (WL). To augment the number of type I genes on the East Lansing (E) map, segregation of the JF-specific allele was followed using preferential amplification of specific alleles (PASA) in polymerase chain reasctions (PCR). Among 15 functional genes that were added to the E map, agrin and mannose-6- phosphate receptor genes were found to occur in oconserved syntenic groups. Using the PCR-based approach, six conserved groups spanning more than 243 centimorgans (cM) in the chicken were syntenic with human and mouse.

Last Modified: 11/28/2014
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